Increased Levels of IL-16 in the Central Nervous System during Neuroinflammation Are Associated with Infiltrating Immune Cells and Resident Glial Cells

Interleukin (IL)-16, a CD4+ immune cell specific chemoattractant cytokine, has been shown to be involved in the development of multiple sclerosis, an inflammatory demyelinating disease central nervous system (CNS). While cells such as T and macrophages are reported producers IL-16, cellular source IL-16 CNS is less clear. This study investigates correlation expression levels with severity neuroinflammation determines phenotype which produce experimental autoimmune encephalomyelitis (EAE) mice. Our data show that significantly increased brain spinal cord tissues EAE mice compared phosphate buffered saline (PBS) immunised controls. Dual immunofluorescence staining reveals IL-16+ lesions likely CD45+ infiltrating or F4/80+ resident CD11b+ microglia GFAP+ astrocytes, but not NeuN+ neurons. suggest cytokine closely pathology evidenced by its glial cells, impacts recruitment activation neuroinflammation.